<jats:title>Abstract</jats:title><jats:p>In the culture broth of the fungus <jats:italic>Clitocybe diatreta</jats:italic> a novel diketopiperazine metabolite, diatretol (<jats:bold>1</jats:bold>), was detected by chemical screening. The structure was established on the basis of <jats:sup>1</jats:sup>H‐ and <jats:sup>13</jats:sup>C‐NMR data and single‐crystal X‐ray analysis. Diatretol exhibits a low antibacterial activity and inhibits the growth germination of <jats:italic>Lepidium sativum</jats:italic>.