Heterologous Expression and Manipulation of Three Tetracycline Biosynthetic Pathways

Angewandte Chemie International Edition
2012.0

Abstract

A very accommodating host: Three tetracycline biosynthetic pathways were overexpressed and manipulated in the heterologous host Streptomyces lividans K4-114. Through the inactivation of various genes and characterization of the resulting biosynthetic intermediates, new tetracycline-modifying enzymes were identified (see scheme).

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