A producer of validamycins, Streptomyces hygroscopicus subsp. linioneus (IFO 12703, ATCC 21431, FERM-P 468) was found to produce additional pseudo-aminosugars besides previously reported valiolamine, valienamine, validamine and hydroxyvalidamine. In this paper, we report the isolation, structural study and aglucosidase inhibitory effects of new minor products of this fermentation, epivaliolamine and deoxyvalidamine.