Tagetes erecta belongs to the Compositae family and is grown as an ornamental plant in China. T. erecta exhibits nematocidal, fungicidal, and insecticidal activity, and its roots have been used in agriculture for nematode control for a long time [1]. Twenty-two naturally occurring compounds with various carbon skeletons were isolated from the flowers of T. erecta by systematic phytochemical investigation, and the structures of the compound were identified by the means of spectroscopic data, including 1H NMR and 13C NMR, and compared with the literature data. Herein we report the results of a thorough phytochemical study on 22 compounds from the flowers of T. erecta by isolation of various fractions of the ethanol extract by silica gel column chromatography. They were -sitosterol (1) [2], -daucosterol (2) [3], 7-hydroxysitosterol (3) [4], lupeol (4) [5, 6], erythrodiol (5) [7], erythrodiol-3-palmitate (6) [8], 1-[5-(1-propyn-1-yl)-[2,2-bithiophen]-5-yl]-ethanone (7) [9–11], -terthienyl (8) [12], quercetagetin (9) [13], quercetagetin-7-methyl ether (10) [14], quercetagetin-7-O-glucoside (11) [15], kaempferol (12) [16], syringic acid (13) [17], gallic acid (14) [17], 3--galactosyl disyringic acid (15) [18], 3--galactosyl disyringic acid (16) [18], 6-ethoxy-2,4-dimethylquinoline (17) [19], oplodiol (18) [20, 21], (3S,6R,7E)-hydroxy-4,7-megastigmadien-9-one (19) [22], palmitin (20) [23], ethylene glycol linoleate (21) [24], and n-hexadecane (22) [18].