A high-performance liquid chromatographic (HPLC) system has been developed for the separation of thirteen Cinchona alkaloids. This system separates into two distinct groups the quinoline alkaloids, which are found predominantly in Cinchona bark, nd the indole alkaloids, which have been found to predominate in the leaves of one species. HPLC has been used to examine the alkaloid content of four plantation samples of Cinchona leaves obtained from different parts of the world. All four samples were found to contain different proportions of alkaloids and although the cinchophyllines were the major alkaloids found in two samples of Cinchona ledgeriana, quinoline alkaloids were also present. However, quinoline alkaloids were the major alkaloids of Cinchona succirubra from Thailand and C. succirubra × C.ledgeriana from Guatemala. In addition, quinamine and 3-epiquinamine were present in the four leaf samples investigated. © 1983.