Literature

Abstract

The iso-migrastatin (iso-MGS) biosynthetic gene cluster from Streptomyces platensis NRRL 18993 consists of 11 genes, featuring an acyltransferase (AT)-less type I polyketide synthase (PKS) and three tailoring enzymes MgsIJK. Systematic inactivation of mgsIJK in S. platensis enabled us to (i) identify two nascent products of the iso-MGS AT-less type I PKS, establishing an unprecedented novel feature for AT-less type I PKSs, and (ii) account for the formation of all known post-PKS biosynthetic intermediates generated by the three tailoring enzymes MgsIJK, which possessed significant substrate promiscuities.

DOI： 10.1021/ja4002635

Post-Polyketide Synthase Steps in Iso-migrastatin Biosynthesis, Featuring Tailoring Enzymes with Broad Substrate Specificity

Journal of the American Chemical Society 2013.0

iso-Migrastatin, Migrastatin, and Dorrigocin Production in Streptomyces platensis NRRL 18993 Is Governed by a Single Biosynthetic Machinery Featuring an Acyltransferase-less Type I Polyketide Synthase

Journal of Biological Chemistry 2009.0

Engineered production of iso-migrastatin in heterologous Streptomyces hosts

Bioorganic & Medicinal Chemistry 2009.0

Iso-Migrastatin Congeners from Streptomyces platensis and Generation of a Glutarimide Polyketide Library Featuring the Dorrigocin, Lactimidomycin, Migrastatin, and NK30424 Scaffolds

Journal of the American Chemical Society 2005.0

Elucidation of the Biosynthetic Gene Cluster and the Post-PKS Modification Mechanism for Fostriecin in Streptomyces pulveraceus

Chemistry & Biology 2013.0

Cloning and characterization of a bacterial iterative type I polyketide synthase gene encoding the 6-methylsalicyclic acid synthase