Three new acetylenic metabolites (1-3) were isolated from the sponge Stelletta sp. collected from Gagu-Do, Korea. On the basis of the results of combined spectroscopic analyses, the structures of these compounds were determined to be an acetylenic acid, its 2-methoxy analogue, and corresponding anhydride, respectively. The new compounds exhibited weak cytotoxicity against a human leukemia cell-line (K562).