<jats:title>Abstract</jats:title><jats:p>Asperamides A (<jats:bold>1</jats:bold>) and B (<jats:bold>2</jats:bold>), a sphingolipid and their corresponding glycosphingolipid possessing a hitherto unreported 9‐methyl‐C<jats:sub>20</jats:sub>‐sphingosine moiety, were characterized from the culture extract of <jats:italic>Aspergillus niger</jats:italic> EN‐13, an endophytic fungus isolated from marine brown alga <jats:italic>Colpomenia sinuosa</jats:italic>. The structures were elucidated by spectroscopic and chemical methods as (2<jats:italic>S</jats:italic>,2′<jats:italic>R</jats:italic>,3<jats:italic>R</jats:italic>,3′<jats:italic>E</jats:italic>,4<jats:italic>E</jats:italic>,8<jats:italic>E</jats:italic>)‐<jats:italic>N</jats:italic>‐(2′‐hydroxy‐3′‐hexadecenoyl)‐9‐methyl‐4,8‐icosadien‐1,3‐diol (<jats:bold>1</jats:bold>) and 1‐<jats:italic>O</jats:italic>‐β‐<jats:sc>d</jats:sc>‐glucopyranosyl‐(<jats:italic>2S</jats:italic>,<jats:italic>2′R</jats:italic>,<jats:italic>3R</jats:italic>,<jats:italic>3′E</jats:italic>,<jats:italic>4E</jats:italic>,<jats:italic>8E</jats:italic>)‐<jats:italic>N</jats:italic>‐(2′‐hydroxy‐3′‐hexadecenoyl)‐9‐methyl‐4,8‐icosadien‐1,3‐diol (<jats:bold>2</jats:bold>). In the antifungal assay, asperamide A (<jats:bold>1</jats:bold>) displayed moderate activity against <jats:italic>Candida albicans</jats:italic>.