The genus Campanula (Campanulaceae) includes around 300 herbaceous species, with bell-shaped blue flowers, used in traditional medicine to treat many diseases and possessing refreshing, antiallergic, antiphlogistic, antioxidant, spasmolytic, antiviral, and antimicrobial properties. Flavonoids are the most important secondary metabolites of the genus, and three anthocyanins were isolated from acetic acid extract of the light blue-purple flowers of C. medium. However, no research on the composition and antiradical properties of C. medium lipids was available before. Owing to their medical properties and health-boosting constituents, nonconventional oils are used in the healthcare industry, which contain bioactive lipidic compounds (polar lipids, sterols, fat-soluble vitamins) apart from triacylglycerols. This investigation analyzed lipid classes, fatty acids, sterols (ST), and tocopherols of C. medium lipids for the first time to indicate the potential nutraceutical and economical utility of C. medium seeds as a source of edible oils. C. medium seeds were found to contain about 9.2% lipids, with neutral lipids (ca. 98.7% of total lipids, mainly triacylglycerols accounting for ca. 96.9% of total neutral lipids) being the highest, followed by glycolipids (0.66%) and phospholipids (0.56%). Linoleic (C18:2) and oleic (C18:1) acids were the major fatty acids, accounting for about 83.1% of total fatty acid methyl esters (FAME), with polyunsaturated fatty acids (PUFA) accounting for 71.4% of FAME. Seven sterol compounds were detected, with β-sitosterol comprising ca. 52% of total sterols. All tocopherol isoforms were present, with α-tocopherol (42.5% of total tocopherols) and γ-tocopherol (ca. 41.3% of total tocopherols) being dominant. C. medium lipids had higher radical scavenging activity (RSA) than olive oil: after 60 min incubation, 35% of DPPH radicals were quenched by C. medium lipids (vs. 9% by olive oil), and 32% of galvinoxyl radicals were quenched (vs. 13% by olive oil).