A new simple aliphatic glucoside, 3-(R)-3-β-D-glucopyranosyloxybutanolide (kinsenoside) with its congeners and a heterocyclic aromatic glucoside, β-D-glucopyranosyl-3-pyridinemethanol (nicoloside) have been isolated from whole Anoectochilus koshunensis plants. Their structures were elucidated from chemical and spectroscopic evidence.