<jats:title>Abstract</jats:title><jats:p>Fourteen alkaloids from <jats:italic>Vinca minor</jats:italic> L. were separated and their concentration determined by high performance liquid chromatography using Separon SGX C18 with a mobile phase of methanol:water (0.02 mol/L NH<jats:sub>4</jats:sub>Cl, pH 8.2 adjusted with Et<jats:sub>3</jats:sub>N). The major alkaloids of the leaves were vincamine (0.13%), 1,2‐dehydroaspidospermidine (0.06%) and vincaminoreine (0.028%). The dominant alkaloid of the root was 1,2‐dehydroaspidospermidine (0.28%). The bisindole alkaloid vincarubine was found only in the leaves (0.007%).