Two new colored metabolites of Streptomyces murayamaensis have been isolated and characterized as 4-hydroxy-3-nitrosobenzamide and its ferrous chelate. These structures have been confirmed by synthesis via a modified Baudisch reaction. A sample of 3-amino-4-hydroxy[2-2H]benzoic acid was fed to S. murayamaensis, affording a sample of the 4-hydroxy-3-nitrosobenzamide ferrous chelate deuterated at C-2 (28.5% enrichment, 3.2% incorporation).