Apart from 17 previously listed fungal taxa producing cyclosporin A and its natural congeners B to Z, several additional and taxonomically diverse strains producing the single novel component [Thr2,Leu5,Leu10] or cyclosporin-like peptolides (eg SDZ 214-103 = [Thr2,Leu5,D-Hiv8,Leu10]) have recently been described. We report here the isolation of two further and novel cyclosporins, [Thr2,Leu5,Ala10] (2) and [Thr2,Ile5]cyclosporin (3), from strains classified as Acremonium luzulae (Fuckel) W Gams and Leptostroma anamorph of Hypoderma eucalyptii Cooke & Harkn, respectively. In both new strains the usual pattern of cyclosporins A to Z is not found. The structure elucidations of 2 and 3 are based on NMR spectroscopy, and biological data (immunosuppressive activity, cyclophilin-binding affinity and antifungal effects) are presented.