As reported in our previous paper, vanoxonin inhibiting thymidylate synthetase (EC 2.1.1.45) was found in the culture broth of Saccharopolyspora hirsuta KG245-CF2. Recently, we found that this strain produces two other antibiotics in a different medium. One of them is identical with nargenicin A1 (CP-47,444) produced by Nocardia argentinensis, and the other is a new antibiotic, I-N-amidino-l-N-demethyl-2-hydroxydestomycin A. In this communication, the isolation and structural elucidation of the new antibiotic is reported.