This study analyzed the alkaloid composition of seven Turbinicarpus species (Cactaceae) cultivated by the Cactus Club of Ostrava and identified by commercial suppliers and comparisons with descriptions. Fresh plants (200–240 g each) were cut, homogenized in 95% ethanol, left overnight at 5°C, filtered, evaporated to dryness, dissolved in 0.1 M HCl and chloroform, and the aqueous phase was basified to pH 10 with concentrated ammonia before chloroform extraction. Alkaloids were identified by GC (cochromatography with reference compounds on DB1 and DB5 columns) and GC-MS (EIMS-70 eV). Seven β-phenethylamines (β-phenethylamine, tyramine, N-methyltyramine, hordenine, mescaline, N-methylmescaline, N,N-dimethylmescaline) and four tetrahydroisoquinoline alkaloids (O-methylanhalidine, anhalinine, anhalonidine, pellotine) were found, often in appreciable amounts. While these alkaloid types are known from other plant families and cactus genera, their presence in Turbinicarpus is novel. The results suggest alkaloid profiles have potential value for infrageneric taxonomy of Turbinicarpus.