The structures of the two natural didcmnms with a modified macrocycle, [Ty?]didcmnin B [2] and [D-Pro4]didemnin B [3], isolated from lhc Aplousobranch ascidian Trididrmnum cyanophorum (Didemnidae) are described. S~uctures were dctcrmincd by FABMS and NMR specuoscopy, absolute configurations by Marfey analysis of the acid hydrolisatcs. Conformational informations arc given and cyloloxic aclivitics against human lymphoblastic leukemia cell lines evaluated.