In our preceding papers, the isolation, physicochemical and biological properties of chlorocarcins A, B and C from Streptomyces lavendulae were described. From the same strain No. 314, an antibiotic complex closely related to chlorocarcins but without chlorine was isolated, containing five components designated saframycins A, B, C, D and E; saframycins B, C, D and saframycin E acetyl derivative were crystalline. Isolation was similar to chlorocarcins but omitting 1 N NaOH counter extraction; further purification used column and preparative thin-layer chromatography. Physicochemical properties (m.p., [α], elemental analysis, UV, IR, NMR, mass) of each saframycin were described. They are soluble in lower alcohols, chloroform, acetone; slightly in ether; insoluble in n-hexane, water; positive Dragendorff reaction, negative Ehrlich and FeCl3 reactions. Antimicrobial spectra showed activity only against gram-positive bacteria, with saframycin A most active and D/E least. While saframycins have similar UV/IR to chlorocarcins, they differ in chlorine content and NMR details (e.g., saframycin A lacks chlorocarcin A's δ1.23 peak; saframycin B has a single methoxy peak vs. two in chlorocarcin B); saframycin C is assumed to be saframycin B's O-methyl derivative. Antibiotics with UV maxima near 270 and 370 nm are unreported, and their properties distinguish saframycins from known antibiotics.