Two previously undescribed alkaloids (1-2), five known alkaloids (3-7) and five cyclodipeptides (8-12) were obtained from an ansatrienin-producing mutant strain Streptomyces sp. XZQH13OE Delta astC. Their structures were elucidated by analysis of the 1D, 2D NMR and ESI HRMS data and by comparison with the reported data. The antibacterial activities of compounds 1-12 were evaluated.Graphical Abstract