Three polyoxygenated bipyrrole alkaloids were isolated from the aerial of Speranskia tuberculata, including two new compounds, speranberculatines B (1) and C (2), along with a known compound, speranskatin A (3). Their structures were identified via NMR-spectroscopic and MS analyses. None of them showed activity in inhibiting the production of NO in LPS-induced RAW264.7 cells.