A new alkaloid, (Z)-2-(1 '-amino-2 '-hydroxyvinyl)benzoic acid (1), along with 11 compounds, including five aporphines, (-)-nuciferine (2), (-)-nornuciferine (3), (-)-asimilobine (4), (-)-anonaine (5), and (-)-roemerine (6); two oxoaporphines, lysicamine (7) and liriodenine (8); two isoquinolones, northalifoline (9) and thalifoline (10) and two steroids, beta-sitostenone (11) and stigmasta-4,22-dien-3-one (12) were isolated from the rhizomes of Nelumbo nucifera. The structure of the new alkaloid was elucidated by chemical and physical evidence.