This study examines the chemical compositions of most common and widely distributed mangrove species Bruguiera gymnorhiza. Here, the metabolic profiling of B. gymnorhiza leaves via gas chromatography-mass spectrometry and an advanced ultra-high performance liquid chromatography-Q-Exactive Orbitrap high resolution mass spectrometry was performed to screen and identify the bioactive chemical constituents. Total of 75 diverse chemical constituents were identified which belong to various classes, including flavonoids, fatty acids, terpenoids, carboxylic acids, phenolic acids, quinones, and alkaloid. Among these metabolites flavonoids, fatty acids and terpenoids were found to be the major component of B. gymnorhiza. Moreover, the compounds which are key drivers of pharmacological actions of plants such as, gallic acid, hispidulin, rhamnetin, embelin and 5-O-methyl embelin were reported. This study is the first report on the HRMS analysis of chemical constituent of B. gymnorhiza and can be considered as comprehensive map of its biologically important metabolites. © 2022 SAAB