Postoperative recoveries of glaucoma patients may be difficult due to excessive growth of human tenon capsule fibroblasts. In this study, we investigated the functional mechanisms of liensinine, an isoquinoline-type alkaloid in lotus seeds, in regulating transforming growth factor beta 1-associated cell development and signaling pathways in human tenon capsule fibroblast cells. Pre-incubation of liensinine was found to suppress transforming growth factor beta 1-induced migration, inhibit autophagy signaling pathway, reduce p38 phosphorylation in human tenon capsule fibroblasts, and downregulate mitogen-activated protein kinase 7 gene, which was then over-expressed in transforming growth factor beta 1-treated human tenon's fibroblast cells. It was shown that mitogen-activated protein kinase 7 overexpression reversed the suppressing effect of liensinine on proliferation, migration, autophagy, and p38 signaling pathways. Thus, our study indicated that liensinine could functionally regulate the transforming growth factor beta 1-induced proliferation, migration, and associated signaling pathways in human tenon's fibroblast cells, possibly through mitogen-activated protein kinase 7 gene. These findings may help to develop suitable therapeutic strategies for postoperative recovery in glaucoma patients.