Through a phytochemical investigation of Abrus mollis Hance, a folk medicinal plant in China, we isolated and identified three undescribed compounds, including two flavonoids and one amides alkaloid, along with nine known from this plant. Their structures were elucidated by analyses of 1D, 2D NMR, HR-ESI-MS, ECD, and DP4+ analysis. Furthermore, we evaluated the hepatoprotective effects of all twelve compounds on D-GalN-induced Brl-3 A cells. According to the results, at a concentration of 25 muM, the cell survival rates were observed to be 71.92+/-0.34 %, 70.03+/-1.29 %, and 69.11+/-1.90 % for compound 2, 4, and 11, respectively. Further experimental studies showed that compound 2 (EC(50) 5.76+/-0.37 muM) showed more significant protective activity than the bicyclol. CI - (c) 2023 Wiley-VHCA AG, Zurich, Switzerland.