A phytochemical investigation on the aerial parts of Gardneria ovata Wall resulted in the isolation and identification of 14 compounds, including three gardneria glycoalkaloids (1-2 and 6), seven gardneria alkaloids (3-5 and 7-10), and four oxindole alkaloids (11-14). The structure of compound 1 was elucidated by means of spectroscopic analysis, including HRESIMS together with 1D and 2D NMR experiments. Compounds 1-2 and 11-14 are the first time reported from of G. ovate, while the compounds 3-4, 6, and 8 are also the characteristic secondary metabolites of the title plant. The chemotaxonomic significance and distribution of these mono-terpenoid indole alkaloids in Gardneria genus are discussed.