Lantana camara is a notorious weed that has various uses in folk medicine. The L. camara leaf extract produces a number of metabolites and some have been shown biological properties. A number of studies reported the biological pharmacological activities of L. camara as well as the toxicity on insect pests. The study of physicochemical characterizations of L. camara crude leaf extract may lead to the prospect of L. camara as potential biopesticides source. Crocidolomia pavonana larvae are one of cabbage insect pest that becomes resistant to chemical insecticides, so alternative botanical insecticides are expected to control more environmentally friendly. The phytochemical analysis of L. camara leaf ethanolic extract was investigated in this study. Subsequently, the physical characterizations i.e. particle size analysis, potential zeta, and FTIR analysis was done. The biological toxicity test using spray application method. The suspension that's sprayed onto the leaf disc area was led to dry and the powder-shaped suspension residues were attached on the leaf surface, then the 3rd instar C. pavonana were exposed on it. The parameters are the mortality of larvae at 24, 48 and 72 hours, the average mortality was then analyzed statistically using probit test to determine the LC50 value. The result showed that the L. camara leaf ethanolic extract of with polydisperse particles (PI = 0.726), particle size = 541.7 nm - 4265.8 nm, and the particles tend to incipient instability (Z = -14mV) and pH=6. The extract had toxicant bioactivity on 3rd instar larvae C. pavonana (80 % mortality at 24h and 100% mortality at 48h and 72h) with LC501.88% at 24 h, 1.21% at 48h and 0.99%) at 72h, considered as a medium toxic category. FTIR-spectra showed O-H, C-H absorptions groups revealed to terpenoids, the absorptions groups of (0-H),(C-H), (C=C), (R-COOH) and C-O-C glycoside linkages to the sapogenins that confirmed the presence of saponins, the absorbance of (-OH) and aromatics ring indicated the presence of steroids and the absorbance of the (C-N) aliphatic amines indicate presence of alkaloids. These compounds present in the phytochemical result of analysis (triterpenoids, saponins, steroids, and alkaloids). Based on the result of physicochemical characterizations are recommended to allow the next study Lantana prospect for new biopesticides to control C. pavonana larvae. © 2020 Author(s).