For the first time, suspension cultures produced tropane alkaloids and were obtained from root explants of Hyoscyamus muticus L. It was shown that to obtain a well-proliferating suspension culture from the plant under investigation, a root explant was used. It is advisable to add 6-benzyladenine (0.5 mg L-1) in addition to α-naphthaleneacetic acid (1.0 mg L-1) to the nutrient Murashige and Skoog medium. Histological studies have shown that suspension cultures of H. muticus L. have a complex structure and are characterized by the presence of nodular structures, which are apparently necessary for synthesis and the accumulation of tropane alkaloids. The use of methyl jasmonate (100 and 200 μМ) made it possible to increase the productivity of the suspension culture of H. muticus L., which forms atropine four times more than in plants and more than 5.5 times the root. © 2022, The Society for In Vitro Biology.