Phytochemical investigation of the fruits of Solanum incanum L. resulted in the isolation of nine compounds, including three steroidal glycoalkaloids (1-3), two neolignans (4-5), two simple phenolics (6-7), one monoterpene glycoside (8), and one coumarin glucoside (9). The structure elucidation of isolated compounds was determined based on NMR and mass spectral data. Compounds 4 and 9 were isolated for the first time from the genus Solanum and their chemotaxonomic significance was discussed.