Objective: There are different geographic origins of Aconiti Kusnezoffii Radixs (AKRs) sold in the market with different quality. This study aims to establish a rapid analysis method to distinguish the different geographic origins of AKRs and to realize the rapid evaluation of their quality. Methods: An ultra-performance liquid chromatography coupled with time-of-flight mass spectrometry (UPLC-Q-TOF MS) method was utilized to acquire the constituents' information of AKRs from different geographic origins. MSE data and Progenesis QI software were employed to identify the chemical constitutes. Principal component analysis (PCA) was applied to comparing MS data to find the chemical markers of AKRs from different geographic origins. Results: Twenty-three components were detected and 17 out of them were identified, including diester-diterpenoid alkaloids, monoester-diterpenoid alkaloids , and amine-diterpenoid alkaloids. Three pairs of isomers were detected and two of them were distinguished by the retention time of standard samples. Thirteen chemical markers were screened out through PCA and orthogonal partial least square discriminant analysis. Through detecting Napelline or isomer of Napelline (m/z 360.2530) and Aconifine (m/z 662.3170), AKRs from inner Mongolia autonomous could be screened. According to the existence of benzoylaconine (m/z 604.3108) and Indaconitine (m/z 630.3159), it could be confirmed that the AKRs are from Xinjiang Uygur autonomous. AKRs that cannot detect compounds above-mentioned could be from Liaoning or Shanxi Province. Conclusions: The chemical profile could be used not only to distinguish the AKRs from different geographic origins but also to identify the true and false of AKRs. This study lays a foundation for the study of efficacy and toxic of AKRs.