A new alkaloid, hositisine E (1) and nine known compounds (2-10) were isolated from the seeds of Ormosia hosieiHemsl. et Wils. Compound 1 was identified on the basis of a combination of UV, IR, NMR, CD and HRESIMS data. The structures of the known compounds were determined as isoprunetin (2), biochanin A (3), ononin (4), 4',8-dimethoxy-7-O-β-D-glucopyranosylisoflavone (5), sphaerobioside (6), ambocin(7), rutin (8), kaempferol-3-rutinoside (9) and narcissin (10). In the anti-inflammatory activity assay, compounds 1-3,7 displayed inhibitory effects against lipopolysaccharide-induced interleukin-6release in RAW264.7 macrophages, exhibiting IC50 values of 19.4 – 58.9 μM. © 2022 ACG Publications.