The sclerotia are also broken and combined with flour during milling. Sometimes sclerotia of similar size to the grain are developed in dry climates that are even more difficult to remove. Ergot alkaloids (EAs) are nitrogen-based chemicals that are classified as indole alkaloids. Ergots are a group of metabolites produced by different fungal species. The bio-synthetic pathway of ergot formation involves different genes and proteins, although proteins involved in the ergot synthetic pathway vary according to different fungal species. Ergot ring formation is the shared process in all species. D-lysergic acid is the prime substrate for the formation of ergopeptines catalyzed by an enzymatic complex known as nonribosomal peptide synthetase. Rapid Alert System for Food and Feed recently published a report showing that mycotoxins are still the leading cause of food hazards. In addition to the toxic effects on human populations, animals are equally affected due to EA contamination. © 2023 Pradeep Kumar, Madhu Kamle, and Dipendra Kumar Mahato.