Arsenic contamination in drinking water is a major public health concern. It is classified as an environmental pollutant that necessitates immediate corrective action. Long-term arsenic exposure may cause hepatotoxicity. The current research focuses on the effectiveness of a methanolic extract of Centella asiatica against sodium arsenate-induced hepatotoxicity in Danio rerio (Zebrafish). Phytochemicals and bioactive compounds in C.asiatica extract were studied using standard procedure and GC-MS analysis. The extract was also assessed for antioxidant and antibacterial properties. The significant scavenging activity of oxygen-free radicals as well as the IC50 values was observed. Furthermore, the ability of C.asiatica (500 mg/g) leaf extract to alleviate hepatotoxicity in Zebrafish pretreated and post-treated with sodium arsenate (100g/l) was observed. In this work, phytochemical examination of C.asiatica leaf extract revealed the presence of bioactive substances such as alkaloids, flavonoids, terpenoids, glycosides, phenolics, proteins, and carbohydrates, which was validated by GC MS analysis of different percentage areas. Importantly, the methanolic extract of C.asiatica demonstrated strong antimicrobial and antioxidant activities. The plant extract, in particular, demonstrated its efficiency by alleviating the effects of arsenate in the liver of zebrafish (both pre-and post-treatment), as demonstrated by histological analysis. The results of histopathological research revealed that methanolic extract of C.asiatica had a considerable lowering effect on sodium arsenate-induced hepatocyte damage in zebrafish. As a result, the inhibitory action of Centella asiatica methanolic extract may be investigated to create effective candidates to fight arsenate toxicity. © 2022 SAAB