Background: The prevalence of diabetes mellitus patients in Central Sulawesi is 2.2%. Diabetes mellitus in the long term with uncontrolled glucose levels can cause various complications, one of which is diabetic nephropathy. Diabetic nephropathy is a complication of DM in the kidneys that occurs due to a decrease in the glomerular filtration rate and can end in kidney failure. Objective: The purpose of the study is suggested evaluate (Jatropha gossypifolia L) ethanol extract of diabetic nephropathy on male white rats (Rattus norvegicus) induced by streptozotocin 40mg/kg BW intraperitoneally with dose variations of 300mg/kg BW, 400mg/kg BW and a dose of 500mg/kg BW. Method: This study used 30 male white rats divided into 6 groups, namely group I as normal control, group II as positive control (glibenclamide), group III as negative control (Na-CMC 0.5%), group IV, V and VI as control. the test group was given ethanol extract of red jatropha leaves with each dose of 300mg/kgBW, 400mg/kgBB, 500mg/kgBW. The data were analyzed using the one way ANOVA test at the 95% confidence level, and continued with the LSD post hoc test. Result: The results of this study indicate that the ethanol extract of red jatropha leaves contains secondary metabolites, namely flavonoids, alkaloids, phenols, saponins, tannins and can reduce blood glucose levels with a value of 108.0mg/dl, urea 18.2 mg/dl, creatinine 0, 23mg/dl and has an effect on the regeneration of renal tubular cells with a damage value of 1 out of a maximum of 2. Conclusion: This research only took place in on the 21st day and surgery was performed because the condition of the test animals showed toxic characteristics with foam in the mouth, dark eyes, limp, not aggressive and not moving due to the toxic effects of ricin poison which is a compound of toxalbumin, so this research has no potential as therapy. diabetic nephropathy. © RJPT. All right reserved.