From the roots ofEurycoma longifolia, seven compounds including beta-carboline-2N-oxide-1-propionic acid(1),9,10-dimethoxycanthin-6-one (2),beta-carboline-1-propionic acid (3), infractine (4), eurylene (5), eurycomanone (6), 14,15-beta-hydroxyklaineanol (7) were isolated. Their structures were determined by means of spectroscopic methods (UV, IR, HR-ESIMS, 1D and 2D NMR). Compound (1) has been isolated for the first time from nature and compound (4) has been isolated for the first time fromEurycoma longifolia.