With the aim of screening more bioactivity metabolites, in this study, two new indole alkaloids, felisindoles A and B (1 and 2), were isolated from the cigar tobacco-derived endophytic fungi Aspergillus felis. Their structures were determined by means of HR-ESI-MS and extensive 1D and 2D NMR spectroscopic data. Interestingly, the anti-fungi activity assay revealed that compounds 1 and 2 showed potential anti-G. cichoracearum (the main pathogen of tobacco powdery mildew disease) activity with inhibition rates of 72.6% (degrees) 5.2, and 61.7% +/- 5.0, respectively, compared to the negative control. However, these rates are lower than that of the positive control (with an inhibition rate of 93.2% (degrees) 5.5).