Three new aromatic polyketides (±)-puniceusone A (1), (±)-2,3-dihydroxypropyl 6-ethyl-2,4-dihydroxy-3-methylbenzoate (2), (5-ethyl-3-hydroxy-2-methyl) phenyl-β-D-glucoside (3), and two new isoquinoline alkaloids puniceusine O (4) and (±)-puniceusine P (5), were isolated from the deep-sea-derived fungus Aspergillus puniceus SCSIO z021. Their structures were determined by spectroscopic analyses. Compound 1 contains an uncommon hydrogenated benzochromene scaffold, 3 and 4 are glycosides, and 5 is unexpectedly composed of a polysubstituted benzyl and an isoquinoline nucleus. © 2022 Elsevier Ltd