This research is focused on the estimation of total bioactive contents and the evaluation of in vitro pharmacological activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the species Atractylis cancellata. The antioxidant activity was assessed by three different techniques. The antibacterial activity was determined using the agar disk diffusion assay against five bacterial strains. Furthermore, the anti-inflammatory activity was evaluated by the ovalbumin method. According to the results, A. cancellata extracts are rich in several classes of secondary metabolites, especially steroids, triterpenoids, flavonoids, and alkaloids. In addition, the tested extracts showed very interesting antioxidant activities in DPPH (2,2-Diphenyl-1-picrylhydrazyl) and FRAP assays and important correlation coefficients between the results of antioxidant activities and total phenolic and flavonoid contents were found. Moreover, all the tested extracts displayed an antibacterial effect at least against three bacterial strains. The petroleum ether extract inhibited the growth of all the tested bacteria in a dose-dependent manner except Escherichia coli ATCC 25922 and it revealed a strong anti-inflammatory activity (81.77 +/- 0.05%). We conclude that A. cancellata could be an important source of natural pharmacological candidates against oxidative stress, inflammatory and microbial diseases.