Neem (Azadirachta indica A. Juss) is a tropical plant that belongs to the Meliaceae family. It has an antifertility effect that could be developed for a male contraceptive candidate. This study evaluated the phytochemicals characteristics of neem leaf ethanolic extracts from Kajhu and Limpok in Aceh Besar Regency, Aceh, Indonesia, through phytochemicals screening, FTIR, and GCMS analysis. The result showed the differences of active compounds of neem leaves ethanolic extract from Kajhu and Limpok. Neem leaves ethanolic extract from Kajhu have more active compounds than from Limpok area, which contains flavonoids, tannins, saponins, alkaloids, steroids. The main compound neem leaves ethanol extract in Kajhu is linolenic acid, while hexadecanoic acid is found in Limpok. Its active compounds serve as an antifertility agent that could disrupt gonadotropin hormone and spermatogenesis. © 2021, Rasayan Journal of Chemistry, c/o Dr. Pratima Sharma. All rights reserved.