Three new benzazepine alkaloids, cirrhobenzazepines A-C (1-3), together with four known alkaloids (4-7) were isolated from the whole plants of Thalictrum cirrhosum. Their structures were elucidated by spectroscopic methods, including extensive 1H, 13C, and 2D-NMR techniques. Compounds 1-7 were tested for their anti-rotavirus activity. The results revealed that compounds 1-7 exhibited potent anti-rotavirus activity with TI valves in the range of 11.9-18.2, respectively. © 2020 The Japan Institute of Heterocyclic Chemistry