Phytochemical investigation of the ethyl acetate extract of the edible macrofungus, Ramaria madagascariensis, has led to the isolation of two new alkaloids (1 and 2). Their structures were elucidated by HR-ESI-MS, IR, and 1D-and 2D-NMR experiments. The antimicrobial activity of 1 and 2 was also tested and evaluated.