Two new indole alkaloid derivatives (1, 2), together with six known indole alkaloids (3 – 8) were isolated from the 70% EtOH/H2O extract of the stem of Nauclea officinalis. Their structures were determined on the basis of extensive analyses of spectroscopic data (IR, MS, 1D- and 2D-NMR). All the isolates were evaluated for their anti-inflammatory activities on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7, and all the compounds showed significant inhibitory activities with the IC50values of 0.82, 6.45, 9.75, 1.34, 3.40, 2.69, 1.58, and 1.96 μm, compared to the positive drug control group aminoguanidine with an IC50value of 1.80 μm, especially compound 1 had the most significant activity. © 2016 Wiley-VHCA AG, Zürich