Three new aporphine alkaloids, fissitungfines C–E (1–3), together with five known analogues (4–8) were isolated from the stems of Fissistigma tungfangense. Their structures were elucidated by extensive spectroscopic methods Among them, fissitungfines C (1) and D (2) are two unusual aporphine alkaloids with a carboxyl group as a substituent at N-6. All isolated compounds were evaluated for their anti-rheumatoid arthritis activities via examining their anti-proliferative effects on synoviocytes in vitro. Fissitungfine E (3) exhibited a significant inhibitory effect on the proliferation of synoviocytes comparable to that of methotrexate. © 2018 Phytochemical Society of Europe