A pair of new alkaloid enantiomers [(+)- and (−)-1] as well as a pair of known enantiomeric analogues [(+)- and (−)-2] were isolated from the fruiting bodies of Ganoderma luteomarginatum. Their planar structures were determined by extensive spectroscopic analyses. The absolute configurations were established by comparison of the experimental and calculated electronic circular dichroism (ECD) or comparison of the experimental and reported specific optical rotation ([α]D). These rare Ganoderma alkaloids have a phenyl-substituted 6,7-dihydro-5H-cyclopenta[c]pyridine skeleton that has only been reported from the genus Ganoderma. The (+)- and (−)-1 were new Ganoderma alkaloids, while (+)- and (−)-2 were isolated from G. luteomarginatum for the first time. Thus, these four isolates could be tentatively determined as chemotaxonomic constituents of G. luteomarginatum. © 2019 Elsevier Ltd