Four new indole alkaloids, kopsininate (1), N-1-decarbomethoxy chanofruticosinic acid (2), methyl N-1-decarbomethoxy chanofruticosinate N(4)-oxide (3) and methyl chanofruticosinate N(4)-oxide (4), along with eleven known compounds were obtained from a Chinese medicinal plant, Kopsia hainanensis, with an activity-guided purification process. The structures of these compounds, 1-15, were established, mainly by spectroscopic analysis. These compounds were evaluated for antibacterial activities against five bacteria, in which compound 2 was the most active against Erwinia carotovora with MIC of 7.8 mu g/ml. Moreover, compounds 2 and 8 were tested for antifungal activities against four plant pathogenic fungi, 2 exhibited potent inhibitory activities against Fusarium oxysporum f. sp. Cubense and F. oxysporum f. sp. Niveum, which was more active than the positive control (mildothane). These results provide promising baseline information for the potential use of this plant and its components in the control of food spoilage. Crown Copyright (C) 2013 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.