Nine new corynanthe-type alkaloids (1-9) and five known analogues (10-14) were isolated from the seeds of Strychnos angustiflora. Their structures were established based on extensive spectroscopic and spectrometric data, and the structure of 1 was confirmed using single-crystal X-ray crystallography. Based on NMR and electronic circular dichroism (ECD) data analyses, the R absolute configuration of the N-4 stereogenic center of 2 was shown to be opposite to that of 1. In addition, the configurations of compounds 4-8 were established by hydrolysis experiments, and the antirhine moieties were determined to be identical to 11, which was confirmed for the first time using single-crystal X-ray crystallography data analysis. Compounds 1, 4, 10, and 11 exhibited analgesic activity, which increased the hind-paw licking latency rates by 56-78% at an intraperitoneal dose of 20 mg kg-1during a hot-plate test in mice. © 2015 Elsevier Ltd. All rights reserved.