Four new compounds, a dibenzylbutane lignin, plasiaticine F (1), an acetylenic glycoside, plasiaticine G (2), an indole alkaloid, plasiaticine H (3), and an ionone derivative, plasiaticine I (4), were isolated from the seeds of Plantago asiatica. The structures of the new compounds were elucidated on the basis of comprehensive analysis of spectroscopic data. Compounds 1-3 were tested for their cytotoxicity, but lacked significant activity.