A new norsesquiterpe alkaloid (1) was isolated from the solid culture of mushroom-forming fungus Flammulina velutipes fermented on rice. The structure of 1 was elucidated by spectroscopic methods. The absolute configuration of C-1 in 1 was determined using the circular dichroism data of their [Rh 2(OCOCF3)4] complex. © 2012 Kai-Shun Bi. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.