A novel natural product indole, alkaloid, named rel-pyricolluminol (1), was isolated from Aspidosperma pyricollum Müll.Arg. together with six known metabolites sitsirikine (2), aparicin (3), ulein (4), stemmadenine (5), lupeol (6), and (3β)-sitoster-3-yl β-D-glucopyranoside (7). These compounds were characterized on the basis of their spectral data, mainly 1D- (1H,13C-DEPTQ) and 2D-NMR (1H,1H-COSY, NOESY, HSQC, and HMBC), and mass spectra (EI-MS and HR-ES-MS), involving also comparison with data from the literature. © 2015 Verlag Helvetica Chimica Acta AG, Zürich.