Two novel alkaloids, with an unprecedented carbon skeleton, pileamartines A (1) and B (2), together with the known alkaloid, julandine (I-a) were isolated from the alkaloidal fraction of Pilea aff. martinii leaves. Their structures were established by spectral data analysis, including MS and 2D NMR. The absolute configurations of 1 and 2 were suggested by comparison of their experimental and calculated electronic circular dichroism spectra. Compound 2 exhibited weak inhibitory activity against the a-glucosidase enzyme with an inhibition of 11% at a concentration of 200 mu M. These compounds were not cytotoxic against several cancer cell lines even at a concentration of 150 mu M. (C) 2018 Published by Elsevier Ltd.