Three new pyrrolizidine alkaloids, nervosine VII (1), nervosine VIII (2) and nervosine IX (3) were isolated from the whole plant extract of Liparis nervosa. Their structures were elucidated by extensive spectroscopic analyses (including 1D, 2D NMR, and HR-ESI-MS) and chemical methods. Compounds 1-3 were evaluated for their cytotoxic activity against A549, MCF-7and H460 human cancer cell lines. (C) 2016 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.