Quite recently, our laboratory reported the isolation and analysis of terpenoid indole alkaloids from Mappianthus iodoides Hand.-Mazz. (Cong et al., 2014). As part of our ongoing chemical investigation of M. iodoides, three new indole alkaloids, mappiodosides H-J (1-3), were isolated from the stems of M. iodoides. Their structures were determined by comprehensive analysis of spectroscopic data including 1D and 2D NMR and HRMS data. All new compounds were evaluated for their cytotoxic activity against four human cancer cell lines, HT-29, MDA-MB-231, BEL-7402 and K-562, but were inactive.